فصلنامه زیست شناسی جانوری تجربی
سال سوم شماره 3 (پیاپی 11، زمستان 1393)

The altered or mutated forms of genes known as proto-oncogenes are responsible for promoting cell growth and uncontrolled cell proliferation. An accumulation of many mutations in different and specific genes،over time is required to cause cancer. The pattern of gene expression، also called molecular signature is unique to a particular class of tumor or tumor cell. This paper describes the latest technique for monitoring the expression of a panel of cancer-specific genes. The PCR technique combines the quantitative performance of SYBR® Green-based real-time PCR is widely used for gene profiling. This technique is cost-effective، easy-to-use، and focuses only on the genes that you desire. In this study the expression of our target genes were quantitatively determined in five human cancer cell lines. We selected gene β-actin as our reference gene. Cells were lysed and the mRNAs were extracted using the RNA Purification Kit and cleaned up with Qiagen RNeasy spin columns. The first-strand cDNA was synthesized according to the High Capacity cDNA Reverse Transcription Kit protocol. RT-PCR were performed with Gene Expression Assays in an AB step one plus Sequence Detection System. Briefly the expression of p53 was high in both breast cancer cell lines، MCF7، T47-D and lung cancer cells، A549. Src expression was higher in prostate cell line، PC3 and lung cancer cells، A549. Meanwhile SKOV3 (ovarian cancer cell line) showed high expression of her-2 gene. The results clearly show that the expression pattern of this panel of genes was unique to almost every cell line examined.

In order to study and compare the morphometric and meristic characteristics of Alburnoides eichwaldii، 38، 37 and 30 specimens of this species in the Rivers of Tajan، Aras and Babolrood were captured، respectively. In total، 25 morphometric characteristics such as total length، fork length، standard length، length of fins etc and 5 meristic characteristics، such as the number of vertebrae، the number of scales on the lateral line، etc. were measured. Based on the results from this study in Tajan River، Aras and Babolrood، the mean weight، 7. 65، 4. 90 and 5. 97 (gr)، and the average total length of 80. 28، 75. 37 and 76. 96 (mm)، fork length 73. 76، 69. 18 and 71. 40 (mm) and standard length 63. 83، 60. 50 and 62. 86 (mm) respectively. Meristic characteristics of Spirlin in Tajan، Aras and Babolrood Rivers are as follows: number of vertebrae in the range of 35-41، 36-41 and 34-40، respectively. The number of scales on the lateral line of the fish in the Three Rivers Spirlin is in the range of 33-54، 40-52 and 34-51 were counted. Results of multivariate statistical techniques (PCA) and (CVA) in the morphometric characteristics of Tajan، Babolrood and Aras Rivers، somewhat detached seen. The meristic characteristics of this Type of fish in the Mentioned Rivers studied، very close together، and there was high overlap in their characteristics.

Despite the significant role of macroinvertebrates، they have not received enough attention in running water ecosystems of Iran. Behesht-e Gomshodeh is one of the most important localities among Protected Areas in Fars Province. The river that originates from the mountains of this area is connected to the Kor River and flows to the Dorudzan Reservoir. This paper presents the results of a study on macrozoobenthos of the Behesht-e Gomshodeh River. Sampling was performed during winter and spring 2012 at three sampling sites. A total of 25 families have been identified، and class Insecta (especially، order Ephemeroptera) had the most variety. In overall، the number of identified taxa in spring was more than winter. The greatest taxon richness (17 families) and more number of sensitive taxa were found at site 1، while the lowest taxon richness (11 families) and more resistant taxa to pollutants were shown at the site 3. Therefore، site 1 could be considered as a more sensitive place.

Geographic information system (GIS)، science and tecnology of georefrence datas analysis، can assimilate many parameters at once and this ability with modelling statistic bases supplies a suitable set of appling ecological models for ecologists. One of these ecological models is habitat model for wildlife species that it is important in fild of managemant and conservation wildlife species. Models predicting the spatial distribution of species and suitability habitat are currently gaining interest. As they often help both in understanding species niche requirements، their use has been especially promoted to tackle conservation issues، such as managing species distribution، assessing ecological impacts of various factors (e. g. pollution، climate change)، risk of biological invasions or endangered species management. Accordingly، we explore the habitat models to introduce the approach to wildlife managers.

The threadfin breams of the genus Nemipterus are commercially important in many parts of the world; therefore، stock parameters such as age، growth and mortality have been examined to manage them properly. The growth parameters of Nemipterus japonicus were studied by using length frequency data of 763 specimens of this species that were collected monthly by trawl net from Khuozestan Coastal during Jun 2005 to May 2006. FiSAT II program was used to again Pauly regression and their equation، to determine the growth parameters. The Pauly equation indicated L∞= 254. 06 for female and 270. 42 for male fishes respectively. ELEFAN I program was used to catch Length- Frequency curve and amount of K parameter، K estimated 0. 684 and 0. 546 in male and female respectively. After gain amount of L∞ and K parameters، by used of this data and FiSAT II program، estimated Natural and Total mortality and with this data Fisheries mortality was calculate. Natural mortality was 0. 8 and 0. 6 and Total mortality calculated، 2. 97 and 1. 99 in male and female respectively. Finally Fisheries mortality was 2. 17 and 1. 39 in male and female respectively.

According to many literatures marsh frog (Rana ridibunda) probably has the potential to become a commercial species which was exploited at least once about 30 years ago in Anzali wetland region. The global decline of amphibians urgently calls for a better understanding of amphibian population dynamics. Study of population size of marsh frog which has not been yet conducted in Gorigol wetland was carried out using the mark-recapture method. The objective of this study was to establish the population size of a population for contribution to the rather limited knowledge of the ecology of the species، which is widespread in Iran. We could not to achieve our main goal (i. e. estimating of population size) due to lack of enough recapture data. But، this study indicated that it is useful to conduct the robust design to increase the recapture probability of individuals. According to our results، a primary period needs to be one week، and within the week، the secondary periods should be the series of two nightly or more samples.

The present study was investigated artichoke extract in drinking water on performance، carcass characteristics and some blood parameters concentrationssuch as Triglyceride، Cholesterol، very low density lipoprotein (VLDL)، low density lipoprotein (LDL) and high density lipoprotein (HDL) in broiler chickens. Experiment wasused by randomized complete block design (RCBD) with 200 broiler chickens consisted of 5 treatments 0، 100، 200، 300 and 500 milligrams artichoke extract per water liter at four replicates per treatment and 10 chickens per replication during 21-35 days of age. Artichoke extract had any significant effect on feed intake، body weight gain، feed conversion ration and carcass characteristics. Artichoke extract had any significant effect on the blood parameters in broiler chickens during 35 day of age. It can be concluded that inclusion extract of artichoke up to 500 milligrams per liter has no beneficial consequences on performance and blood serum fat constituents.